FastQCFastQC Report
Wed 25 May 2016
SRR1780917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757541
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT167282.2081973120926786No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG24870.3282990623609811No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC16440.21701795678385727No Hit
CATATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT13760.18164033365850826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10980.14494264996878056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG23950.055.8321170
GCGTCTA200.00694744552.507031
CGACTTC352.086773E-550.0000948
GCCGTCT30600.049.30073546
CCGTCTT30350.049.2455247
TATGCCG30350.049.0083943
CGTGCGA506.200935E-848.9968610
CGTCTTC30900.048.9321348
CGTATGC30750.048.71553841
ATCTCGT29900.048.69895637
CTCGTAT30550.048.57940339
TCGTATG31150.048.53941740
TCTCGTA30950.048.5170338
ATGCCGT31050.048.2480444
TGCCGTC31300.048.0863345
GTATGCC31000.047.75542
ACGATCT30400.047.65512534
GACGATC30950.047.5997133
CGATCTC31150.047.29721535
GATCTCG31400.047.1528836
Produced by FastQC (version 0.11.5)