FastQCFastQC Report
Wed 25 May 2016
SRR1780916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169360
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2623415.490080302314595No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG6250.3690363722248465No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA150.002221881269.9979369
CGTTATG150.002221881269.9979312
TGGTCGC1400.052.4984549
TTTCGCC200.00694266852.49844729
CGTCTCT200.00694266852.49844731
CATGTCG200.00694266852.49844768
AAAGACG200.00694266852.49844723
GTCGCCG1500.048.9985551
GCCGTAT1500.048.9985554
CTCGGTG1450.048.27443344
TCGCCGT1550.047.41795352
CGTATCA1550.047.41795356
GTAGATC1550.047.41795338
TCGGTGG1550.047.41795345
GATCTCG1500.046.66528741
CGGTGGT1600.045.93614246
CGCCGTA1600.045.93614253
TAGATCT1650.044.5441439
ATCTCGG1600.043.74870742
AGATCTC1600.043.74870740
Produced by FastQC (version 0.11.5)