FastQCFastQC Report
Wed 25 May 2016
SRR1780916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169360
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT158609.364666981577704No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG23481.3863958431743033No Hit
CATATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT13220.7805857345299952No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC13090.7729097779877184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10330.6099433160132263No Hit
ATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGTCTT4000.23618327822390173Illumina PCR Primer Index 4 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC3520.20784128483703354No Hit
CTTATACACATCTCCGCGCCCACGAGACTTCTACCAATCTCGTATGCCGT2630.1552905054322154No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCACTCTCGTATGCCGT1700.10037789324515824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT150.002221658169.999734
CGTCTTA150.002221658169.999748
ACGTCAC150.002221658169.999743
CGGTACA150.002221658169.999751
TACGTAA150.002221658169.999710
AACACGT150.002221658169.999737
CGAATGC453.8198777E-1062.2219641
ATCTCGA401.0599251E-861.24974437
AGGGGGG20500.060.95096270
AATATCG700.059.9997536
TATCGTA800.056.87476338
GACTTAT252.3852495E-455.99976326
ATATCGT750.055.99976337
GCCGACT252.3852495E-455.99976346
TTGCGAT252.3852495E-455.99976316
GCCGTAT252.3852495E-455.99976346
ATCGTAT653.6379788E-1253.8459339
ACAACGA200.006941972852.49978319
GCAACGT200.006941972852.49978340
TCGTGTT200.006941972852.49978334