FastQCFastQC Report
Wed 25 May 2016
SRR1780915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47726
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2892260.600092192934675No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG7201.5086116582156477No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT680.14247998994258895No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG670.140384695972845No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG630.13200352009386918No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG620.12990822612412523No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG510.10685999245694171No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480.10057411054770984No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG150.00221181870.0000135
ATCATTA1600.056.87500459
TCATTAA1600.056.87500460
GTATCAT1600.054.68750457
TATCATT1600.054.68750458
CATTAAA1700.053.52940861
CCGTATC1650.053.03030455
CGTATCA1650.053.03030456
GCCGTAT1650.053.03030454
ACTCCTA200.0069114652.50000429
CCGCATC200.0069114652.50000469
TCTCGGT1600.052.50000443
CTCGGTG1550.051.93548244
AGATCTC1700.051.47058540
CGCCGTA1700.051.47058553
GTAGATC1600.050.31250438
TAGATCT1600.050.31250439
ATCTCGG1700.049.41176242
ATTAAAA1850.049.1891962
TCGGTGG1650.048.7878845