FastQCFastQC Report
Wed 25 May 2016
SRR1780915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47726
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1772737.14327620165109No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG27235.70548547961279No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT15083.1597033063738844No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC14693.07798684155387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12772.6756903993630305No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT3520.7375434773498721RNA PCR Primer, Index 15 (95% over 22bp)
CTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT3480.7291623014708963No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC2610.5468717261031723No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATATCGTATGCCGT1900.3981058542513515No Hit
CTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1810.37924820852365587No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1540.32267527134056906No Hit
CTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC1430.29962703767338555No Hit
ATTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1390.29124586179440975No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGACGT1120.23467292461132297No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC1090.22838704270209112No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGACAATCTCGTATGCCGT1060.22210116079285921No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAGCCAATCTCGTATGCCGT1050.22000586682311526No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG980.20533880903490762No Hit
CTTATACACATCTCCGAGCCCACGAGCCGCTTGCCAATCTCGTATGCCGT970.20324351506516367No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC880.18438586933746803No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG850.17809998742823618No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCAGT720.15086116582156475No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCATCTCGTATGCCGTC720.15086116582156475No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCACTCTCGTATGCCGT720.15086116582156475No Hit
TATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCT660.13828940200310105RNA PCR Primer, Index 15 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG630.13200352009386918No Hit
CTTATACACATCTCCGAGACCACGAGACGCTTGCCAATCTCGTATGCCGT610.12781293215438128No Hit
TCTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCG610.12781293215438128No Hit
CATATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT580.12152705024514938No Hit
CTTATACACATCTCCGAGCCAACGAGACGCTTGCCAATCTCGTATGCCGT570.11943175627540543No Hit
CAAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT560.11733646230566148No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCAAATCTCGTATGCCGT560.11733646230566148No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCTATCTCGTATGCCGT540.11314587436617358No Hit
CTTATAAACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT520.10895528642668566No Hit
CATAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT490.10266940451745381No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG301.00337274E-770.0000136
AATCTCC150.00221181870.000019
CTTTACA150.00221181870.000011
AAATCGT150.00221181870.0000137
GCAAGAA150.00221181870.0000156
GCTAGAC150.00221181870.0000129
CCAAAAT150.00221181870.0000134
CACCGAG207.851091E-570.0000112
CTCTCCG150.00221181870.0000110
TATCGAA150.00221181870.0000138
GAAAAAC150.00221181870.0000160
CAATCTA207.851091E-570.0000135
ACCGAGC207.851091E-570.0000113
AGTATGC150.00221181870.0000141
GACAATA207.851091E-570.0000133
AGAGACG150.00221181870.0000123
GAACAGC150.00221181870.000016
CTTAATA207.851091E-570.000012
TCACCGA207.851091E-570.0000111
AAAATCG150.00221181870.0000136
Produced by FastQC (version 0.11.5)