FastQCFastQC Report
Wed 25 May 2016
SRR1780914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295738
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3568512.066423658779055No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG8250.2789631362895536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2300.045.64897554
TCGGTGG2200.044.5423345
TCTCGGT2200.042.95153443
TCGCCGT2450.041.43267452
CTCGGTG2300.041.0840844
CGGTGGT2400.040.8373846
CGTATCA2500.040.59715756
GGTCGCC2450.040.00396350
GAGTCGT350.001253645640.0039638
TGGTCGC2400.039.37890249
GGTGGTC2500.039.20388447
CCGTATC2600.039.03572555
ATTGGCG459.189119E-538.88616637
CGCCGTA2550.038.4286853
GTATCAT2650.038.29920657
GATCTCG2400.037.91401341
TTAGAGT654.843605E-737.7024154
GTCGCCG2600.037.6960451
TAGATCT2450.037.14025539
ATCATTA2650.036.98479559
Produced by FastQC (version 0.11.5)