FastQCFastQC Report
Wed 25 May 2016
SRR1780914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295738
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT211577.15396736300374No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG31181.0543115866070643No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC23680.800708735434743No Hit
CATATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT17960.6072942942739858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14890.5034861938607822No Hit
ATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGTCTT5510.18631356132793214TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC4410.149118476489325No Hit
CTTATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT3490.11800986007885357No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG150.002224480369.99036429
TTACGAA150.002224480369.99036431
TAAGACG207.924299E-569.99036443
CGTCTAC453.8380676E-1062.22417448
AGGGGGG28500.061.89667570
CACGTAT308.363211E-658.33516739
ATCGTAT800.056.87678539
ATTCTCG252.3877526E-456.00176236
GCCGTCA1450.053.10511846
TTCGGTG200.00694609452.50164811
TTACGCT200.00694609452.50164816
GTCGGAC200.00694609452.50164852
CCGTCTT37700.051.34113347
TATGCCG40300.051.32047743
CGTCTTC37900.051.2549148
TGCCGTC39750.051.2468945
GCCGTCT38500.051.1834346
ATGCCGT40250.051.12339444
CTTGAAA39050.051.0899557
ATCTCGT40550.050.92638437
Produced by FastQC (version 0.11.5)