FastQCFastQC Report
Wed 25 May 2016
SRR1780913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372079
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG367759.883653740200334No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG8230.2211895860825255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTG150.002224429369.994368
CGCGTGT150.002224429369.994367
CCTCGCA200.00695057552.4957664
GTTGGGC200.00695057552.49576641
GGCTACC200.006954271352.4887121
GTGCGCG451.6836311E-646.6629074
GCCGTAT1450.043.45061554
TGGTCGC1650.042.43222849
ATCTCGG1700.041.1731542
CTCGGTG1800.040.8410244
TCGGTGG1800.040.8410245
TCGCCGT1550.040.64734652
GTCGCCG1650.040.30519551
CGGTGGT1750.040.00752646
TGCGCGT350.001255171839.9967735
GATCTCG1800.038.88575441
TCTCGGT2000.035.0018843
TGAACGT501.7090744E-434.9971775
AATGCGT400.002418293434.9971776
GAACGTA501.7090744E-434.9971776
Produced by FastQC (version 0.11.5)