FastQCFastQC Report
Wed 25 May 2016
SRR1780913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372079
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT202725.448305333007237No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG31550.8479382066711638No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC20680.555795946559736No Hit
CATATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT16410.44103537152056416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14200.3816393830342481No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT12750.34266916434413125No Hit
ATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGTCTT4240.11395429465247972RNA PCR Primer, Index 40 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTT150.002223464470.00201438
ACGTATG207.92045E-570.00201440
TCGAAAG207.92045E-570.00201440
GCCCGAC207.92045E-570.00201431
AGGGGGG27600.062.77354470
ATGACCG352.969864E-760.00172829
TGCCGTA650.059.23247545
ATCGTAT900.058.33501439
CTCGAAA252.3885877E-456.00161739
CCGACTC452.7021088E-854.43137733
CGACTCT452.7021088E-854.43137734
CGTATGC37600.052.78077341
GCCGTCT35650.052.72241646
CCCGTCC200.006943875552.50856857
TCGTCCA200.006947568652.5015116
TACTCGA200.006947568652.501512
TAAGACG407.4690615E-752.5015143
GATTGTC200.006951263652.4944537
CCGTCTT35750.052.4770347
TCGTATG38000.052.409440