FastQCFastQC Report
Wed 25 May 2016
SRR1780912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247957
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2823811.388264900769085No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG5440.2193928786039515No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTTA200.00694302952.5049939
GCCGTAT1400.050.01484754
TCGCCGT1350.049.2738852
CGGTGGT1450.048.27071446
CTCGGTG1400.047.5045244
TGGTCGC1400.047.49493849
TCGGTGG1500.046.66169445
GATCTCG1500.044.33754741
GTCGCCG1500.044.32860651
CCGTATC1600.043.76298555
AGATCTC1600.043.75415840
ATCTCGG1600.041.56645242
CGTATCA1700.041.18869456
TCTCGGT1800.040.83721543
CGCCGTA1650.040.31499553
GTAGATC1650.040.3068638
GGTGGTC1900.036.8381847
GTGGTCG1950.035.89361248
GAGTAAC400.00241466135.00332611
TAGATCT1900.035.00332639
Produced by FastQC (version 0.11.5)