FastQCFastQC Report
Wed 25 May 2016
SRR1780912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247957
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT168726.804405602584319No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG24240.9775888561323132No Hit
CATATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT13730.5537250410353408No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC13630.5496920837080622No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9590.38676060768601006No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCCGTGCATCTCGTATGCCGT3730.1504293083074888No Hit
ATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGTCTT3410.13752384486019753RNA PCR Primer, Index 16 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGCCCGCCGTGCATCTCGTATGCCGT3070.12381178994745057No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC2870.11574587529289353No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT150.002223501769.9947632
AGACCGA301.0215081E-769.9947625
TGCCGTA150.002223501769.9947645
GACCGAC252.8356844E-669.9947526
AGGGGGG26150.061.58810870
CGACGTG352.967936E-759.99550629
ATCGTAT352.967936E-759.99550639
CCGTGCT308.364515E-658.32896831
CAACTCG308.364515E-658.32896836
GCCGTAT308.364515E-658.32896846
AAGCCGT800.056.8707444
CGTCTTC30900.053.80941448
GCCGTCT31450.053.74796346
TATGCCG32250.053.7169143
CCGTCTT31250.053.5319947
TCGTATG32900.053.5066340
CGTATGC32450.053.4936841
TCTCGTA32750.053.21738438
CATCTCG32450.053.17012836
ATGCCGT32500.053.08833344