FastQCFastQC Report
Wed 25 May 2016
SRR1780911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569404
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240514.2238902431314145No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT252.3896236E-456.0018275
AACGCGC200.006949347452.5017135
TCGCCGT950.044.2119752
GCCGTAT950.044.2119754
CCGTTGA350.001255575939.9977950
CGCCGTA1100.038.18306753
CGGTGGT1200.037.917946
TGGTCGC1200.037.917949
CTCGGTG1300.035.0011444
GTCGAAA400.002419066434.99806611
GTGGTCG1450.033.7942148
CCGTATC1350.033.70480355
TCTCGGT1400.032.5010643
TCGGTGG1400.032.5010645
GTCGCCG1305.456968E-1232.3059151
GCCGTTG703.4870063E-530.00097849
CGTATCA1700.028.8244756
GGTGGTC1950.028.71888547
GGCCGTA500.00720312428.0033721
CGTAGTG808.66121E-526.25085654
Produced by FastQC (version 0.11.5)