FastQCFastQC Report
Wed 25 May 2016
SRR1780911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569404
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT140082.460116191667076No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG20040.35194694803689475No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC13060.22936263180448327No Hit
CATATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT11680.20512676412529596No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9340.164031162408413No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAGT150.002225001669.99464443
AGGGGGG19850.059.0686870
TCGTTCG252.3909134E-455.9957120
CCGTCTT24500.053.14345647
ATGCCGT25150.053.0178144
GCCGTCT25000.052.78059446
CGTCTTC24950.052.605848
TATGCCG25350.052.5995343
CGTATGC25650.052.5347141
TCGTCGG200.006947519352.50520711
GTCGTCG200.006947519352.50520710
TGCCGTC25550.052.46634745
TCGTATG26100.052.29944240
ATCTCGT25750.051.6510737
CATCTCG25750.051.6510736
CTCGTAT25900.051.35193339
TCTCGTA26050.051.19059838
GTATGCC26250.051.0627642
GTCTTCT26600.048.8163449
CTTGAAA28050.048.6636557
Produced by FastQC (version 0.11.5)