FastQCFastQC Report
Wed 25 May 2016
SRR1780910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231110
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2990012.937562199818267No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG9330.40370386396088437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCACG150.00222275769.999141
GAACAAT150.002224666369.98398645
GGGTCTA252.3871716E-455.999311
CGAACAA200.006939437752.51071544
GAAGTCG200.006939437752.51071553
AAGTCGC200.006939437752.51071554
CGTGTTA200.006945382352.49934833
TTCTGCG200.006945382352.4993482
TGCGCAA200.006951330752.4879885
CGCGTGT200.006951330752.4879887
CCCCGTA305.8699556E-446.66609249
CCCCCGT350.001253763739.99950448
AACTATG350.001253763739.99950451
TTGGGCG350.001253763739.9995042
TCACGAG350.001256435839.98219325
CAGCGTG459.191469E-538.8799939
GCCGTAT1650.036.0679754
TCGCCGT1850.034.06100552
CGTATCA1650.033.94632356
TCGGTGG1950.032.300345
Produced by FastQC (version 0.11.5)