FastQCFastQC Report
Wed 25 May 2016
SRR1780910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231110
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT188008.134654493531219No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG28671.240534810263511No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC19160.8290424473194582No Hit
CATATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT16970.7342823763575786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15250.6598589416295271No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT4650.20120289039851155RNA PCR Primer, Index 15 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC3340.14451992557656526No Hit
CTTATACACATCTCCGCGCCCACGAGACTAATGTCTATCTCGTATGCCGT2650.11466401280775387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTT301.02138074E-769.9928647
ACCGTCC207.919238E-569.992868
AGGGGGG24250.063.69812870
TAACTCG452.6884663E-854.4506736
GCCGACT407.463368E-752.49464446
CCGAGTT200.00694784752.49464444
GCCGTCT30850.052.4095646
CCGTCTT30650.052.40900847
CGTCTTC30800.052.39235748
TATGCCG31450.052.29990843
CTGCTTG30550.052.156254
CTTGAAA31250.052.08595757
ATGCCGT31550.052.0232244
TGCCGTC31300.051.991545
GTATGCC31600.051.8301542
TCGTATG32350.051.72152340
CGTATGC32050.051.65957641
GCTTGAA31500.051.4503356
CTCGTAT32400.051.42563239
GTCTTCT31550.051.36879349