FastQCFastQC Report
Wed 25 May 2016
SRR1780909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200979
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3101015.429472730981844No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG7480.37217818777086165No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTA150.00222244669.998261
GATCATA252.832885E-669.998251
GGGTCTA308.356292E-658.3318821
CAGTAGT308.356292E-658.3318821
CAGTTGT252.3865695E-455.99861
ACTGCCA200.00694441752.4986952
GGTGTCG200.00694441752.4986941
GGTGTCA200.00694441752.498691
GTGTCGG200.00694441752.4986942
GAACGAG200.00694441752.498696
CAGCTTT200.00694441752.498691
TCGGTGG1900.051.5776645
GTCGCCG1750.049.99875351
ATCTCGG1850.049.18796542
GATCTCG1850.049.18796541
CTCGGTG1800.048.60989844
GCCGTAT1800.048.60989854
AGATCTC1750.047.99880640
TCGCCGT1850.047.2961252
TCTCGGT1850.047.2961243
Produced by FastQC (version 0.11.5)