FastQCFastQC Report
Wed 25 May 2016
SRR1780909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200979
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT182689.089506863901203No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG28101.398156026251499No Hit
CATATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT16210.806551928310918No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC13390.6662387612636146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12300.6120042392488767No Hit
ATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGTCTT4560.22688937650202262RNA PCR Primer, Index 31 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC3810.18957204484050572No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGAGATATCTCGTATGCCGT2930.1457863756909926No Hit
CTTATACACATCTCCGCGCCCACGAGACCCCGAGATATCTCGTATGCCGT2910.14479124684668548No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCCGAGATATCTCGTATGCCGT2160.1074739151851686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGG150.00222006570.017171
CAGTTAC150.00222006570.017171
GATCATA301.0182521E-770.017171
GTCTGCC150.00222225869.9997542
CACGAAT150.00222225869.9997539
TAAGATC150.00222225869.9997562
GCCGTAT150.00222225869.9997546
AGGGGGG25100.062.33045670
CAGAGCT308.342915E-658.3476451
ACGAGAT553.6379788E-1157.27252630
CGTATGA800.056.87479441
TGTATTC252.3863174E-455.99980523
CGTCTGC252.3863174E-455.99980541
AAGCCGT509.749783E-1055.99980544
ACCCGAG252.3863174E-455.99980528
CTTTATA750.055.99982
TGACGTC653.6379788E-1253.84596345
CGTCTTC31200.053.0607148
CCGTCTT30850.052.86853447
TCTCGTA33450.052.83986738
Produced by FastQC (version 0.11.5)