FastQCFastQC Report
Wed 25 May 2016
SRR1780908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186761
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3502118.75177365724107No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG9640.5161677223831528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTCG150.002220563670.01124650
TGCTCTT200.006938556752.50843412
ACTTTTC200.006938556752.50843449
TTTTCGC200.006938556752.50843451
TTGGCGG200.00694591152.49437739
GGTACAC200.00694591152.4943772
CGAGCAA200.00694591152.49437739
CGGTTGG305.8620464E-446.67416447
TGTGCGC305.869792E-446.6616673
CGTATCA1650.044.5406856
TCGCCGT1750.043.99528552
GCCGTAT1700.043.23066354
GTCGCCG2000.042.00674451
CGCCGTA1850.041.61716553
GATCTCG1950.041.2776341
TGGTCGC1800.040.83989749
GATCCTA350.001252080240.0064241
CGAGCCA350.001252080240.00642416
GTGCGCG350.001253730639.9957164
CGGTGGT1850.039.7361146
Produced by FastQC (version 0.11.5)