FastQCFastQC Report
Wed 25 May 2016
SRR1780908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186761
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT2094111.2127264257527RNA PCR Primer, Index 14 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG34261.8344301005027814No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC21941.1747634677475491RNA PCR Primer, Index 14 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT18020.9648695391436113No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16370.8765213293996069No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC4810.2575484174961582RNA PCR Primer, Index 14 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACACAGTTGCATCTCGTATGCCGT4660.24951676206488507RNA PCR Primer, Index 14 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGTCTT4640.24844587467404863RNA PCR Primer, Index 14 (96% over 26bp)
CTAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT1880.10066341473862317No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTAT150.00222184470.0010716
CGAGGAC150.00222184470.0010738
ATGCCGG150.00222184470.0010744
GCCGTAA150.00222184470.0010746
ACTGGTA150.00222184470.0010715
TAAGACG150.002224205569.9823343
AGGGGGG26350.062.014770
GGTGTTC252.3888148E-455.98586743
CGTATGA653.6379788E-1253.8325641
TTGCGAT200.006935194652.51486650
CGAGTCC200.00694254752.500867
ATTGCTC200.00694254752.500854
GTGTCAG200.00694990552.4867441
CGGTAAG200.00694990552.48674429
CTCGTAA950.051.5797439
ATCGTAT950.051.5797439
GCATATC950.051.5797435
CGTCTTC34650.051.3139248
CCGTCTT34800.051.29388847
GCCGTCT34950.051.1738946
Produced by FastQC (version 0.11.5)