FastQCFastQC Report
Wed 25 May 2016
SRR1780907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228682
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2878012.58516192791737No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG8910.38962401938062463No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTG150.002222288670.0026254
GTATGCC308.367713E-658.3227611
GGTACAT200.006937914552.5134541
CGGTGGT2100.048.33514846
GCCGTAT1850.047.29907254
TCGGTGG2150.047.21107545
TGGTCGC2100.046.6684249
TTCAACG305.874718E-446.6582128
CTAAGCG305.874718E-446.658219
GATCTCG1950.046.6582141
GTCGCCG2150.043.9551451
TCTCGGT2150.043.94552643
TCGCCGT2100.043.33495752
CGTATCA2150.042.3271756
ATCTCGG2150.042.31791742
CTCGGTG2400.042.28444
CGCCGTA2100.041.6682353
GTAGATC2300.041.08849338
CGTAAGG350.001253429140.00153
GATACCT350.001254778239.9927528
Produced by FastQC (version 0.11.5)