FastQCFastQC Report
Wed 25 May 2016
SRR1780907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228682
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT176757.729073560665029No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG28971.2668246735641633No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC17500.7652548079866365No Hit
CATATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT16420.7180276541223184No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14380.6288208079341618No Hit
ATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGTCTT5280.23088830778111089RNA PCR Primer, Index 38 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC3880.16966792314217996No Hit
CTTATACACATCTCCGCGCCCACGAGACATTTATCTATCTCGTATGCCGT2890.1263763654332217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAC252.8330287E-670.00284645
TCTGTCG207.922045E-569.987538
ATATCGT401.0581061E-861.27929737
AGGGGGG25450.060.1006770
TCTGCCG252.389075E-455.9900343
GTCTGCC252.389075E-455.9900342
ATTAAGC200.006937826552.5136216
TATGCCA200.00694383452.50213244
CTGCCGT200.00694383452.50213244
CCGACTT407.4556556E-752.50213247
TCAGTCG200.00694984552.4906469
AGCGTCA200.006955859752.479173
ATCTCGT32850.052.23197637
CCGTCTT31450.052.19607547
TCGTATG33000.052.19535440
CGTCTTC31750.052.13244248
TCTCGTA32850.052.1253838
GCCGTCT31750.052.03360746
TATGCCG31950.052.02516643
CGTATGC32450.052.00116741