FastQCFastQC Report
Wed 25 May 2016
SRR1780906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117397
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140318.231300629487976No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG6150.5238634718093308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT207.898293E-570.0012747
AAGTGGA150.002219750870.00127418
GTCCGTA150.002223509669.97143625
AACTGGC252.3920089E-455.953329
GTGGTCC200.00692435852.52335754
GCCGTAT1400.052.52335754
CTCGGTG1400.052.5009644
GCTATAT200.006936054752.5009573
AGGTATC200.006947765652.4785835
TACTGAT200.006947765652.4785823
AGAGCGC200.006947765652.4785838
TCCGTAA200.00695949252.4562226
CGGTGGT1450.050.73385246
TCGCCGT1450.050.71220852
CGTATCA1400.050.02224356
CCGTATC1400.050.02224355
GTATCAT1400.050.02224357
GTCGCCG1500.049.02180551
TGGTCGC1500.049.02180549
TCGGTGG1450.048.31795545
Produced by FastQC (version 0.11.5)