FastQCFastQC Report
Wed 25 May 2016
SRR1780906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117397
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT62765.3459628440249745No Hit
CATATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT62255.302520507338348No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG19051.6226990468240245No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10770.9173999335587792No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC6180.5264189033791323No Hit
CATAAACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT5630.47956932459943613No Hit
CACTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC4280.3645749039583635No Hit
CAAATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT2800.23850694651481721No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT2690.229137030758878No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC2460.2095453887237323No Hit
CATATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCGT1570.1337342521529511No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCCG150.002214444670.0435110
ATCGTAT252.8218328E-670.0136539
ATATCGT150.00221819670.0136437
GCGATCG150.00221819670.0136436
TGGTTAA150.002221952269.98379534
AGACGCA150.002225712969.953992
TACGCGA150.002225712969.9539931
GTACGCG150.002225712969.9539930
AGGGGGG18200.065.91817570
CGAGACC501.4551915E-1163.01228315
ATATACA9050.062.997242
CATATAC9000.062.9585841
CTCTCGT453.8016879E-1062.23435237
CTCGTAA401.0544682E-861.26193639
AGCCGTC401.0581061E-861.23582545
CGACTTC401.0581061E-861.23582548
TCACCGA352.9401417E-760.03729611
CGTATGA700.059.98611541
CGCGATA352.9575494E-759.98611533
CGTCTTA308.344909E-658.3198348