FastQCFastQC Report
Wed 25 May 2016
SRR1780905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656214
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG253663.8655072887807944No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG7340.11185375502503755No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT200.00694595852.50908738
CGGACCC200.00695224152.4970869
TGCGCGT305.879158E-446.6640745
CGCTACG350.001255247440.00082816
GATCGGC501.7087118E-435.0033935
GTCGCCG1350.033.7018351
GCCGTAT1154.0017767E-1133.48150654
TGACGTC450.00430508631.11412437
TGTACGT450.004306691731.11175512
TAGACGT450.00430829831.1093835
TCGCCGT1251.05501385E-1030.80298652
CGACGAA1451.8189894E-1128.96611868
CGGTAAG500.007210104327.99844628
TGGTCGC1653.6379788E-1227.57422649
CGCCGTA1455.9299055E-1026.55429653
CGATAGA700.001225301525.0024237
TCGGTGG1703.754394E-922.64580345
GACGAAA1905.620677E-1022.1057269
GCGATAG1003.9285788E-421.00203536
TCTCGGT1859.946234E-920.81124143
Produced by FastQC (version 0.11.5)