FastQCFastQC Report
Wed 25 May 2016
SRR1780905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656214
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT75731.154044259951784No Hit
CATATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT68521.0441715659830482No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG19030.28999686078017234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13160.20054433462254692No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC9480.14446506779800494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG23850.056.94899770
CAGTCGA200.006948080452.5050366
ACGTACC200.006948080452.5050353
TGCCGTC28000.051.24709745
CGTATGC28350.051.23557341
GCCGTCT27600.051.22898546
CCGTCTT27150.051.1757447
TCTCGTA27950.051.09223638
CTCGTAT28050.051.03486639
CGTCTTC27650.050.89095348
ATGCCGT28150.050.849744
TCGTATG28800.050.67807440
ATCTCGT27700.050.6688637
TATGCCG28400.050.6485443
CGATCTA352.0862468E-550.0009835
ACGATCT28200.049.89079334
GTATGCC28850.049.6159142
GACGATC28600.049.0706533
GATCTCG29200.048.4256136
CGATCTC29550.048.0889335
Produced by FastQC (version 0.11.5)