FastQCFastQC Report
Wed 25 May 2016
SRR1780904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379896
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG241916.367795396634869No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG6840.1800492766441342No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC350.001255148639.99714334
CGGTGGT1550.038.38940446
TCGGTGG1550.038.38940445
TGGTCGC1550.038.38435449
CTCGGTG1750.034.0020544
GATCTCG1750.034.0020541
CGTATCA1450.033.79958756
GCCGTAT1450.033.79958754
ATCTCGG1800.033.05754542
GTCGCCG1700.032.9431651
GACTATA751.487655E-632.6686331
TCTCGGT1900.031.31767343
ATCTCGA450.004303597431.11298444
TGCCTGA450.004306371331.10888949
GTGGTCG2050.029.02231848
AGATCTC1950.028.71589740
GTAGATC1950.028.71211638
TCGCCGT1850.028.38382552
CGCCGTA1850.028.38382553
TCGAGTC500.007206902427.9988
Produced by FastQC (version 0.11.5)