FastQCFastQC Report
Wed 25 May 2016
SRR1780904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379896
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT68411.8007559963779558No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT67641.7804872912586602No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCG18490.4867121527997136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11440.3011350474866806No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC8470.22295575631225387No Hit
CATAAACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCCGT6480.17057299892602187No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTCTACCAATCTCGTATGCC5370.1413544759618422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGT308.371762E-658.33048237
AGGGGGG18650.057.05565670
GCCGTCA900.054.4417846
CCGTCTT20850.052.70725647
CGTATGC22500.052.57520741
TCCGCGA200.00694258252.511261
CGGGTAG200.006949815452.49743310
CCGGGTA200.006949815452.4974333
TCGTATG22800.052.34393340
TGCCGTC22150.051.8259145
GCCGTCT21550.051.80721746
CGTCTTC21350.051.80074748
TATGCCG22400.051.7162243
CTCGTAT22400.051.5599839
TCTCGTA22150.051.3518938
GTATGCC22600.051.25855642
ATCTCGT21950.051.18200737
ATGCCGT22450.051.13335844
AATCTCG22500.050.5530836
TCTTCTG21700.050.48805250