FastQCFastQC Report
Wed 25 May 2016
SRR1780903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44324
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2774362.59137261979966No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9972.249345726919953No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT1180.2662214601570255No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG800.1804891255301868No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG690.1556718707697861No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC650.14664741449327678No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550.12408627380200343No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480.10829347531811208No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450.10152513311073008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG1200.058.33333270
TGGTCGC2050.056.34146549
GGTGGTC2000.056.00000447
TGTGTAG2200.055.68181635
CTCGGTG1950.055.64102644
AGTGTGT2150.055.3488433
TCGGTGG2100.054.99999645
CGGTGGT2100.054.99999646
GTGGTCG2100.054.99999648
CAGTGTG2100.054.99999632
TCGCCGT2050.054.63414852
CGCCGTA2050.054.63414853
GTAGATC2250.054.44444738
TAGATCT2200.054.09090839
TCTCGGT1950.053.84615343
TATCATT1950.053.84615358
GCAGTGT2150.053.72093231
GCCGTAT2150.053.72093254
AGATCTC2150.053.72093240
GTGTAGA2350.053.61702336