FastQCFastQC Report
Wed 25 May 2016
SRR1780903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44324
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT816118.41214691814818No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT797918.001534157567008No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG24455.516198899016334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16603.745149354751376No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC8151.838732966338778No Hit
CATAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT7561.705622236260265No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC5331.2025087988448697No Hit
CAAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT3250.7332370724663839No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT3210.7242126161898745No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC3000.6768342207382005No Hit
CATATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT2300.5189062358992871No Hit
CTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1400.3158559696778269No Hit
TCATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG1400.3158559696778269No Hit
CTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1370.3090876274704449No Hit
ATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT1350.30457539933219024RNA PCR Primer, Index 15 (95% over 22bp)
CAAAAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT1130.2549408898113889No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATATCGTATGCCGT1100.24817254760400687No Hit
AAACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCTT1000.2256114069127335RNA PCR Primer, Index 15 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCTAGCCAATCTCGTATGCCGT880.19853803808320547No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGACGT880.19853803808320547No Hit
CTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC770.1737207833228048No Hit
CCTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCG750.16920855518455014No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGACAATCTCGTATGCCGT670.15115964263153145No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGACAATCTCGTATGCCGT640.14439130042414947No Hit
TCTTATACACATCTCCGCGCCCACGAGACGCTTGCCAATCTCGTATGCCG590.13311073007851276No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATATCGTATGCCGT590.13311073007851276No Hit
CACTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC580.13085461600938542No Hit
TATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTCT570.1285985019402581RNA PCR Primer, Index 15 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCC570.1285985019402581No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCATCTCGTATGCCGTC540.12183015973287609No Hit
CATATACACATCTCCGAGCCCACGAGCCGCTTGCCAATCTCGTATGCCGT530.11957404566374877No Hit
CAATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGTC530.11957404566374877No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCAGT530.11957404566374877No Hit
CATATACACATCACCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT520.11731793159462142No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGACGT500.11280570345636674No Hit
CATATACACATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGA470.10603736124898476No Hit
CATATACACATCTCCGAGCCCACGAGACGCTAGCCAATCTCGTATGCCGT460.10378124717985741No Hit
CATATACACATCTCCGAGCCAACGAGACGCTTGCCAATCTCGTATGCCGT450.10152513311073008No Hit
CATATACAAATCTCCGAGCCCACGAGACGCTTGCCAATCTCGTATGCCGT450.10152513311073008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAAGC500.070.0000124
AGAAGCT500.070.0000125
TGATTGA252.7961323E-670.0000155
CTGATTG252.7961323E-670.0000154
TCTGATT252.7961323E-670.0000153
TCCGAGA455.456968E-1270.0000113
GAGACCA500.070.0000116
ACTAATA150.002210772670.02
TAAACAT150.002210772670.05
GCTAGCA150.002210772670.029
GCGGCTG150.002210772670.049
TAAAAAC150.002210772670.03
TATAAAC150.002210772670.03
ACTCTCG301.0017175E-770.036
GAATGAC150.002210772670.042
TCTATAC150.002210772670.01
ACTTCAG150.002210772670.050
TCAGCGT150.002210772670.03
CGAATGA150.002210772670.041
GTCTTAA207.844908E-570.049
Produced by FastQC (version 0.11.5)