FastQCFastQC Report
Wed 25 May 2016
SRR1780902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177030
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3004416.97113483590352No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9510.5371970852397899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC1600.045.937549
GCCGTAT1600.043.7554
CGTATCA1650.042.42424456
GTATCAT1650.042.42424457
GATCTCG1650.042.42424441
TCGGTGG1750.042.045
TCGCCGT1700.041.1764752
GTCGCCG1700.041.1764751
CTCGGTG1800.040.83333644
AGATCTC1650.040.30303240
CTTATAG350.00125289340.034
TATACCG350.00125289340.06
GTGGTCG1950.039.4871848
GTAGATC1700.039.1176538
ATCTCGG1800.038.8888942
CGCCGTA1850.037.83783753
TCTCGGT1950.037.69230743
GGTGGTC2050.035.8536647
CGGTGGT2050.035.8536646
CCGTATC2000.035.00000455