FastQCFastQC Report
Wed 25 May 2016
SRR1780902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177030
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT87174.924024176693216No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT85624.836468395187256No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCG25971.4669829972321076No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17040.962548720555838No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC9900.5592272496187087No Hit
CATAAACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT7340.4146189911314466No Hit
CACTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC6890.3891995706942326No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCC3450.19488222335197425No Hit
CAAATACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT3300.18640908320623623No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATTTCGAATCTCGTATGCCGT3200.18076032310907755No Hit
CATATACACATCTCCGCGCCCACGAGACCATTTCGAATCTCGTATGCCGT1910.10789131785573067No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTT207.9134494E-569.99406447
AGGGGGG24050.063.73680570
ATATACA13550.061.2125242
CATATAC13700.059.52051
TGACGTC800.056.8701845
ATATCGT800.056.8701837
CGAATCA509.749783E-1055.99525534
TTCGATC252.3864942E-455.99525532
TGCCGTA509.749783E-1055.99525545
CATCTCG452.6879206E-854.4398336
TATGACG900.054.4398343
ATGACGT900.054.4398344
CGAATCT30900.054.0245534
CCGTCTT28700.054.0198147
AATCTCG30850.053.99866536
TCGAATC31250.053.9794233
GCCGTCT29200.053.93378446
TATGCCG31300.053.89319643
CGTCTTC28750.053.80413448
TGCCGTC30900.053.79803545