FastQCFastQC Report
Wed 25 May 2016
SRR1780901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392269
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311047.929252630210391No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9920.25288768676596923No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAT150.002223615870.001531
CGTGAAC200.006951542652.49445728
AGTGATC200.006955048552.4877628
CCGAGAG350.00125383640.00597413
CGGTAGG654.858375E-737.6931329
CGAAATG654.863232E-737.68832470
GCCGTAT1950.035.89364254
CTCGGTG2050.035.85901644
GGCAGTA400.002417237335.0007671
TCTTGGA601.2143253E-535.0007672
TGGTCGC1900.035.00076349
GTCGCCG1900.034.99630451
TCTCTGG400.002418749734.9963047
TCGGTGG2250.034.2229745
CGGTGGT2050.034.14708746
TCGCCGT2000.033.24648752
GCTCGGT652.1038617E-532.3001626
ATCGACC552.9872658E-431.82293712
GCTGACC552.991739E-431.81482324
GATCTCG2100.031.67139841
Produced by FastQC (version 0.11.5)