FastQCFastQC Report
Wed 25 May 2016
SRR1780901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392269
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT84462.153114316961065No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT81262.0715376438107524No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCG21960.5598199194940207No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17270.4402591079080937No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC10670.27200721953557383No Hit
CATAAACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCCGT7260.18507707720977187No Hit
CACTTATACACATCTCCGAGCCCACGAGACCTGACCGAATCTCGTATGCC7060.17997853513787732No Hit
CATATACACATCTCCGAGCCCACGAGACCTGCCCGAATCTCGTATGCCGT4270.10885387323494847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGC401.3460522E-1070.0089241
CGACTTC301.0225267E-770.048
AGGGGGG24100.061.72986270
CGTCTTA950.058.94737248
ATATCGT900.058.34077537
GAGCTAG252.3845761E-456.0214271
GCCGTCA2400.055.41666446
AATATCG900.054.4513936
ATATACA14850.054.1949352
TACGTTC200.00694163452.5133957
TATGCCG31550.052.3613343
CCGTCTT29000.052.2586247
CGTATGC31700.052.0097941
GTATGCC31900.052.0062742
GCCGTCT29600.051.79054346
CTTGAAA29700.051.74720457
CGTCTTC28650.051.6753948
TCGTATG32200.051.63701240
ATCTCGT31600.051.62050637
ATCGTAT950.051.58552639