FastQCFastQC Report
Wed 25 May 2016
SRR1780900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397787
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263816.631941214770719No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG7230.18175556265036313No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTCG150.00222270770.0089335
GTCGCCG1200.052.50009551
TCGCCGT1150.048.6957452
GCCGTAT1150.048.6957454
GATCTCG1100.047.73336441
CGGTGGT1300.045.76931446
ATCTCGG1150.045.65225642
TGGTCGC1350.044.0686149
AGATCTC1200.043.75557740
CTCGGTG1200.043.7500844
TCTCGGT1300.040.3846943
TTGTCCG350.001254767940.00007231
TCGGTGG1450.038.62075845
GCGAACT400.002417516435.00006528
CGGTCGT400.002417516435.00006510
GTAGATC1650.033.9437238
TTTAGCG652.100557E-532.3077526
TAGATCT1650.031.82223939
GGTCATA552.989929E-431.8182411
CCGGTCG552.989929E-431.8182419
Produced by FastQC (version 0.11.5)