FastQCFastQC Report
Wed 25 May 2016
SRR1780900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397787
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT76041.9115757930751887No Hit
CATATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT74791.8801519406114329No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCG19310.4854356728600985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12960.32580250234421937No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC6690.16818045838602066No Hit
CATAAACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCCGT6210.15611369903993846No Hit
CACTTATACACATCTCCGAGCCCACGAGACCGCCGTGCATCTCGTATGCC4720.11865646690314163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA501.4551915E-1162.9995548
ATCGTAT750.060.66622539
ATATCGT750.060.66622537
GCCGACT601.8189894E-1258.33291646
TGCCGAC601.8189894E-1258.33291645
AGGGGGG23000.057.3763670
CGTCTAC252.3892144E-455.999648
AACGCCG252.3892144E-455.999627
CGACGTG452.6977432E-854.44405729
GCCGTCT25250.053.2273446
TATGCCG27100.052.69334843
CCGTCTT25250.052.6728947
TAGACGC200.006948909752.4996225
CGTCTTC25600.052.49962248
CCCGTTC200.006948909752.49962222
TCTCACG200.006948909752.4996228
ACGAAGG200.006948909752.4996222
CGTATGC27650.052.15152441
TCTCGTA27750.051.9635938
GTATGCC27550.051.95969842