FastQCFastQC Report
Wed 25 May 2016
SRR1780899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215808
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2651712.287310943060497No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9280.43001186239620404No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTC150.00222250769.99953523
TGTCCCA308.368957E-658.3194340
TTGCGTT252.3867494E-455.9996318
TTGTCTT200.006938242452.5118251
GGTCTCG200.006944606552.4996542
GGGTATT200.00695097552.48748860
GCCGTAT1950.050.2677354
TCGCCGT2050.049.52334252
GATCTCG2050.047.8045641
GTAGATC2150.047.20899238
GGTCTAA305.862219E-446.6771771
ATCTCGG2100.046.66635542
CATTGAA451.6821468E-646.6555526
TGAACCG305.875633E-446.6555445
AGATCTC2100.046.6555440
GTCGCCG2200.046.14675551
CGTATCA2050.046.1079456
CTCGGTG2250.045.1108144
TCTCGGT2350.044.68055743
CGGTGGT2450.044.2956946
Produced by FastQC (version 0.11.5)