FastQCFastQC Report
Wed 25 May 2016
SRR1780899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215808
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT78983.659734578884935No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT76043.523502372479241No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCG22911.0615917852906287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15730.7288886417556347No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC8590.3980389976275207No Hit
CATAAACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT7030.32575252075919336No Hit
CACTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC5750.2664405397390273No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT3230.1496700771055753No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCC2960.13715895610913403No Hit
CAAATACACATCTCCGAGCCCACGAGACGCCAATTCATCTCGTATGCCGT2710.12557458481613287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC150.00222227470.0013938
AATCTCG353.6943675E-970.0013936
GACGACA301.0201984E-770.0013926
CTTTCGC150.002224317869.98517629
AGGGGGG20700.062.899870
AGACGAC401.0615622E-861.25121725
AGCGTCA352.968918E-759.987293
GTATTCT308.356154E-658.3344949
ATCGTAT308.356154E-658.3344939
ATATCGT308.356154E-658.3344937
GAGACGA601.8189894E-1258.3344924
ATATACA12200.057.3648952
CATATAC12250.056.5856671
CGTCTTA509.749783E-1056.00111448
TGACGTC1000.056.00111445
CACGAAT252.3864371E-456.00111439
TGCCGTA252.3864371E-456.00111445
CGTATGA1150.054.77100841
CGAATGC452.6942871E-854.4329141
TCTCGTA26600.054.34318538
Produced by FastQC (version 0.11.5)