FastQCFastQC Report
Wed 25 May 2016
SRR1780898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157836
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3033219.2174155452495No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG10770.6823538356268531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGT150.0022207870.0044350
GCGGGTC150.0022207870.004436
CATCTTG150.00222357469.98225429
CTCGTAA200.006930543652.519971
GGATAAC200.006930543652.5199746
ACGGCAT200.006930543652.5199745
CGCAATC200.00693924152.50332655
CGGAACA200.006947946752.4866928
CACGGTT200.006947946752.4866918
CAACGGA200.006947946752.4866925
AACTTGA200.006947946752.4866917
CGTTGAG200.006947946752.4866930
CCGGATA200.006947946752.4866924
GCACGGT200.006947946752.4866917
ACGGAAC200.006947946752.4866927
ATCTTGC200.006947946752.4866930
ATGCCCG200.006947946752.4866933
GCCGTTG200.006947946752.4866928
CCGCATG200.006947946752.4866922
ATCGAAT200.006947946752.4866926
Produced by FastQC (version 0.11.5)