FastQCFastQC Report
Wed 25 May 2016
SRR1780898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157836
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT91555.800324387338756No Hit
CATATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT91535.799057249296738No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCG25451.6124331584682836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18891.196811880686282No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC14030.8888973364758357No Hit
CACTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC8560.5423350819838313No Hit
CATAAACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT8430.5340986847107124No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT3880.2458247801515497No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCC3730.23632124483641248No Hit
CAAATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT3670.2325198307103576No Hit
ATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGTCTT2200.13938518462201271RNA PCR Primer, Index 15 (95% over 23bp)
CATATACACATCTCCGCGCCCACGAGACTAATGTCTATCTCGTATGCCGT2040.12924808028586635No Hit
CTAATACACATCTCCGAGCCCACGAGACTAATGTCTATCTCGTATGCCGT1660.10517245748751869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC353.6834535E-970.0060245
TACCAGG150.002220580670.0060258
TGACGAC150.002220580670.0060245
GACGACT150.002220580670.0060246
GCCGTAT150.002220580670.0060246
GCACTAG150.002220580670.006021
TGCCGTA150.002220580670.0060245
AAGCCGT301.0201984E-769.9838444
ATGACGA150.002223374369.9838444
AGGGGGG24950.064.1137370
CATATAC11850.061.735271
ATATACA12250.060.29092
GCAGCGT308.342509E-658.3383521
AGGACGC252.3874207E-455.98707216
CGTATGA700.054.98730541
TAACTCG452.6884663E-854.43187336
TCACCGA850.053.53401611
GCCGCTC200.006929921552.5211648
CTAGAGC200.00693861852.5045173
TAGAGCC200.00693861852.5045174
Produced by FastQC (version 0.11.5)