FastQCFastQC Report
Wed 25 May 2016
SRR1780897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474519
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG352257.4233065483152405No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG10440.22001226505155747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2250.051.333154
CGTATCA2300.050.21716756
CGCCGTA2300.050.21716753
CCGTATC2450.048.5712155
CGGTGGT2600.047.1102146
GTGGTCG2550.045.2891448
TCGCCGT2500.044.79979752
TCTCGGT2600.044.41819843
TGGTCGC2650.043.58011249
GTCGCCG2600.043.0721951
TCGGTGG2900.042.23673645
CTCGGTG2800.041.2454744
GGTGGTC3000.039.66230847
GGTCGCC2850.039.2939350
ATCTCGG3000.038.4957742
GTAGATC3050.037.87267338
GATCTCG3100.037.25789341
TAGATCT3200.036.0973939
GTATCAT3400.033.97043657
AGATCTC3600.032.0865740