FastQCFastQC Report
Wed 25 May 2016
SRR1780897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474519
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT103192.1746231447002122No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT91781.9341691270528685No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCG25790.5434977313869412No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.4082028327632824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC11450.2412969765172733No Hit
CATAAACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCCGT9940.20947527917744074No Hit
CACTTATACACATCTCCGAGCCCACGAGACCCCGAGATATCTCGTATGCC8280.17449248607537315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG28200.059.6939170
CATATAC15600.053.6232531
TATGCCG35200.051.20337343
CGTATGC35450.051.05048841
TCGTATG36250.050.98606540
ATGCCGT35100.050.95042844
GCCGTCT33600.050.82935346
GTATGCC35400.050.61750842
CTCGTAT36250.050.6051439
TGCCGTC35200.050.4079845
CCGTCTT33250.050.31715447
CGTCTTC33550.050.18019548
TCTCGTA36700.050.17542338
ATATACA17050.049.678762
ATCTCGT36950.049.45696337
TATCTCG36950.049.45696336
CTGCTTG34400.048.53840654
GATATCT38250.048.0405234
AGATATC39150.047.5619533
ATATCTC38350.047.56020435