FastQCFastQC Report
Wed 25 May 2016
SRR1780896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336927
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291308.64578974080439No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9870.29294179451335156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACA252.3891535E-455.99738734
CTCGCCG200.00694499552.5053519
GCGCACC200.00694907452.4975510
CGTTTGC200.00694907452.4975510
TGCGTTT200.00694907452.497558
TCTATCC200.00694907452.497553
GCTATAC352.08305E-550.005091
TGCCGGC305.870507E-446.67141719
GATCCTA659.978976E-943.081311
CGAGAAT503.4711065E-642.00427214
GGGTCTA350.001253877840.004071
CGGTGGT1650.038.18570346
TCGGTGG1700.037.06259545
GCGGTAG400.00241580735.00356345
GTAAACG400.00241580735.00356320
TCGCCGT1600.034.99836752
GTCGCCG1800.034.99836751
GTTCGCA400.002417567234.99836735
TTCCAGG400.002417567234.99836725
AGTTCGC400.002417567234.99836710
Produced by FastQC (version 0.11.5)