SRR1780896_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780896_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336927
Sequences flagged as poor quality	0
Sequence length	76
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	8708	2.5845361161319813	RNA PCR Primer, Index 14 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	8185	2.429309613061583	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCG	2292	0.6802660516966583	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1777	0.527413950202863	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC	1204	0.3573474372786984	RNA PCR Primer, Index 14 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCC	788	0.23387855529536072	RNA PCR Primer, Index 14 (95% over 21bp)
CATAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	697	0.2068697373615056	No Hit
CAAATACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	375	0.11130007390324906	RNA PCR Primer, Index 14 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACACAGTTGCATCTCGTATGCCGT	367	0.10892567232664643	RNA PCR Primer, Index 14 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	15	0.0022237974	69.997925	3
CGACATT	15	0.0022237974	69.997925	14
GCAGCGT	15	0.0022237974	69.997925	1
AGGGGGG	2360	0.0	62.740498	70
TGCCGTA	45	3.8380676E-10	62.22037	45
GCCGTAT	35	2.9702278E-7	59.99822	46
CTCGAAA	25	2.3889539E-4	55.998337	39
TCGAAAG	25	2.3889539E-4	55.998337	40
AAGCCGT	65	3.6379788E-12	53.84456	44
ATATACA	1330	0.0	52.89317	2
TAACGTA	20	0.00694861	52.49844	38
ATAGAAC	20	0.00694861	52.49844	5
ATGTCCC	20	0.00694861	52.49844	41
AACTGGT	20	0.00694861	52.49844	3
GGGTTGC	20	0.00694861	52.49844	36
TGTACAG	20	0.00694861	52.49844	31
TGCCGAC	80	0.0	52.49844	45
TATGCCG	3060	0.0	52.26969	43
GCCGTCT	2820	0.0	52.250217	46
CCGTCTT	2785	0.0	52.15285	47