FastQCFastQC Report
Wed 25 May 2016
SRR1780895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184098
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2850515.483601125487512No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG9940.5399298199871808No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG150.002216867670.0402444
GCGTCTA150.002216867670.040241
CGATAAC150.002216867670.0402443
CGGGTAT150.002224050269.98315428
GTCGTTA150.002224050269.98315462
TCCGTCT150.002224050269.98315436
GGACCGA150.002224050269.98315418
CGCGTCT252.3885847E-455.9865267
CGGTCTC200.00692704252.53017844
GGACCCA200.006934495652.515952
TCACGAT200.00694195552.5016346
TACCTAG200.00694195552.5016367
CCGGGTA200.006949420652.48736627
GGTCGTT200.006949420652.48736661
GGCACGT200.006949420652.4873665
TGGACCC200.006949420652.48736651
GACCGGG200.006949420652.48736627
TTCCGGG200.006949420652.4873668
ATTCCGT200.006949420652.48736634
GTTCCGG200.006949420652.4873667
Produced by FastQC (version 0.11.5)