FastQCFastQC Report
Wed 25 May 2016
SRR1780895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184098
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT86184.681202403067932No Hit
CTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT86154.679572836206803No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCG24371.3237514801898989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17690.9609012591119946No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC10590.5752371019782941No Hit
CATAAACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT9340.5073384827646146No Hit
CACTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC6970.37860270073547786No Hit
CAAATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT3770.20478223554845787No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCCGT3680.19989353496507298No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTTATCTATCTCGTATGCC2990.1624134971591218No Hit
CATATACACATCTCCGCGCCCACGAGACATTTATCTATCTCGTATGCCGT1970.10700822388075915No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCAA150.002224118469.982611
GCCGTAT150.002224118469.9826146
AGGGGGG23600.064.5492970
CATATAC12250.060.5563851
ATATACA12750.058.7305072
CGTATGA1050.053.3345741
GTTCCGC200.00691980652.5440652
AGTTCCG200.006927253752.52977451
CGTCAAC200.006934707552.51549548
GCAACCG200.006942167452.50122541
CGTCTTC31350.052.48757648
GTACCAG200.006949633452.4869581
CGTAAGG200.006949633452.4869583
CTGGATG200.006949633452.4869583
TCTCGTA32150.052.147438
GCCGTCT31250.052.0670746
CCGTCTT30800.052.03252447
CTCGTAT32300.052.01359639
ATCTCGT32250.051.98570637
ATGCCGT31750.051.9083644
Produced by FastQC (version 0.11.5)