FastQCFastQC Report
Wed 25 May 2016
SRR1780894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301071
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG255668.49168468567215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACAT350.001254293440.01
CGGTGGT901.03682396E-1038.88888546
GCGCTGG459.185725E-538.88888515
GCCGTAT654.8541915E-737.69230754
TGGTCGC852.566594E-937.05882349
CGGGGTT400.002416607735.035
GTGCGCC400.002416607735.034
CGCTTAG400.002416607735.019
ATCTCGG904.485628E-934.99999642
GTAGATC904.485628E-934.99999638
CTCGGTG904.485628E-934.99999644
GATCTCG904.485628E-934.99999641
TCGGTGG904.485628E-934.99999645
GTCGCCG957.605195E-933.15789851
CGTATCA751.4869947E-632.66666856
CCGGAAA552.9882466E-431.8181818
TAGATCT1001.2538294E-831.49999839
GATCGGG450.00430346131.11110932
TGGCGCA450.00430346131.11110954
CCCAACG450.00430346131.11110921