FastQCFastQC Report
Wed 25 May 2016
SRR1780894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301071
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGT155815.175191233961424No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCG28420.9439633840522668No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC13220.4390990829405688No Hit
ATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCCGTCTT5390.17902753835474025TruSeq Adapter, Index 1 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGTCCGCGATCTCGTATGCC4610.15312002816611364No Hit
CTTATACACATCTCCGCGCCCACGAGACTGTCCGCGATCTCGTATGCCGT3280.10894440181884009No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3190.10595507372015238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC252.835899E-670.048
CCCGTGT150.002223305270.059
GACGACT150.002223305270.046
AGGGGGG20150.060.09925570
ACCGCGA252.3881937E-455.99999631
CGACACT252.3881937E-455.99999623
TGTCCGA452.6946509E-854.44444329
CCGTGTG200.006947079752.560
CGTCTTA407.465751E-752.548
CGCGAAC608.367351E-1152.533
CGGATTT200.006947079752.534
TTGCGTT200.006947079752.518
GCGGACC200.006947079752.544
GTCTTCA1200.052.549
CGTCTTC27300.051.79487248
GCCGTCT27300.051.79487246
CCGTCTT27300.051.28205547
TATGCCG28000.051.2543
TCTCGTA28300.050.83038738
GATCTCG28100.050.8185136