FastQCFastQC Report
Wed 25 May 2016
SRR1780893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45988
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2678658.24562929459859No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG1690.3674871705662347No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730.15873706184221972No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710.15438810124380273No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG550.1195964164564669No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540.11742193615725842No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500.10872401496042446No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480.10437505436200747No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACATA150.002211299370.067
CATTAGA150.002211299370.055
CCATTAG207.8480225E-570.054
TCCATTA150.002211299370.053
ATCATAA150.002211299370.059
TGTGGGC207.8480225E-570.03
CTATGGT150.002211299370.027
TTTATGC150.002211299370.07
GTCGGGC150.002211299370.010
TCGGGCG150.002211299370.011
GGTGTCG200.006909851552.541
CATAAAA200.006909851552.561
TGTAGAT851.8189894E-1245.29411737
GTGTAGA901.8189894E-1242.77777536
CGTGTAG901.8189894E-1242.77777535
GTCGCCG855.4569682E-1141.1764751
TTACGTG855.4569682E-1141.1764732
TACGTGT953.6379788E-1240.52631433
CCGTATC701.8600986E-840.00000455
GCCGTAT701.8600986E-840.00000454