FastQCFastQC Report
Wed 25 May 2016
SRR1780893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45988
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT1716837.33147777681135No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG30026.527789858223884No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC12352.685483169522484No Hit
ATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTCTT5131.115508393493955Illumina PCR Primer Index 1 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC4270.9285030877620248No Hit
CTTATACACATCTCCGCGCCCACGAGACACGTGACGATCTCGTATGCCGT4180.9089327650691484No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3700.8045577107071411No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGCTCTCGTATGCCGT3230.702357136644342No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATATCGTATGCCGT3100.6740888927546316No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGCCGATCTCGTATGCCGT2950.6414716882665044No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGACGT1980.43054709924328083No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCAGT1850.40227885535357055No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGACGATCTCGTATGCCGT1740.37835957206227716No Hit
CTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTC1470.3196486039836479No Hit
CTTAAACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT1330.28920587979472906No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGA1210.26311211620422714No Hit
CTTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG1050.22832043141689135No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGCTCTCGTATGCCG1010.2196225102200574No Hit
CTTATACACATCTCCGAGCCAACGAGACACGTGACGATCTCGTATGCCGT1010.2196225102200574No Hit
ATTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT990.21527354962164041No Hit
TCTTATACACATCTCCGCGCCCACGAGACACGTGACGATCTCGTATGCCG970.21092458902322342No Hit
CTTATACACATCTCCGAGACCACGAGACACGTGACGATCTCGTATGCCGT960.20875010872401495No Hit
TATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTCT950.20657562842480645Illumina PCR Primer Index 1 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTC860.18700530573193008No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGAATGCCGT850.18483082543272158No Hit
CCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG830.18048186483430462No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTAAGCCGT790.17178394363747065No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGAACTCGTATGCCGT770.16743498303905366No Hit
CATATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT740.1609115421414282No Hit
TTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGTC720.15656258154301123No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGTGACGATCTCGTATGCCGT700.15221362094459426No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCC650.1413412194485518No Hit
CTTATACACATCTCCGAGCACACGAGACACGTGACGATCTCGTATGCCGT630.1369922588501348No Hit
CTAATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT590.12829433765330087No Hit
CTTAATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCG590.12829433765330087No Hit
CTTATACACATCTCCGAGCCCACGAGCCACGTGACGATCTCGTATGCCGT590.12829433765330087No Hit
TCTTATACACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCAG540.11742193615725842No Hit
CTTATAAACATCTCCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT530.11524745585804992No Hit
CTTATACACATCTCAGAGCCCACGAGACACGTGACGATCTCGTATGCCGT520.11307297555884144No Hit
CTTATACACATCACCGAGCCCACGAGACACGTGACGATCTCGTATGCCGT480.10437505436200747No Hit
CTTATACACATCTCCGAGCCCACGAGACACGTGACGATCACGTATGCCGT480.10437505436200747No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCT150.002211299370.052
CTTTACA150.002211299370.01
ACGTCTA150.002211299370.047
TATGCTT150.002211299370.053
TAAGACG150.002211299370.043
AAGACGT150.002211299370.044
AGACGTC150.002211299370.045
AGACGAA150.002211299370.032
AACTAAA150.002211299370.032
GTAAGAC150.002211299370.042
CGTAAGA150.002211299370.041
GTGAAGA150.002211299370.031
CGTGAAG150.002211299370.030
TCTTTAT301.0025542E-770.01
GAGACGA600.070.031
ACGTGAA150.002211299370.029
CTATTAT150.002211299370.01
AGGGGGG19900.066.48241470
GACACGA700.065.0000126
CGAACTC550.063.63636835
Produced by FastQC (version 0.11.5)