FastQCFastQC Report
Wed 25 May 2016
SRR1780892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314013
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3268010.407212440249289No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAATTG200.00694735552.50000412
GCCGTAT850.049.41176654
CGTTAAA305.872841E-446.66666417
GCCGTTA305.872841E-446.66666415
TCGCCGT1000.045.50000452
TGGTCGC1000.045.50000449
CGGTGGT950.044.2105346
TCGGTGG1050.043.33333645
CTCGGTG901.8189894E-1242.7777844
GTCGCCG1100.041.36363651
GGCCGTT350.001254375240.00000414
GGTCTAA556.683671E-638.1818161
ATCTCGG1003.237801E-1035.00000442
CAGTTGT400.002416764935.0000041
CAGTTAC400.002416764935.0000041
CGGCTTA400.002416764935.00000425
GATCTCG1003.237801E-1035.00000441
GTAGATC1102.3646862E-1135.038
CGCCGTA1350.033.70370553
TCTCGGT1253.6379788E-1233.643
Produced by FastQC (version 0.11.5)